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To achieve this goal we genetically transformed Arabidopsis thaliana with an ACS (Aminocyclopropane-1-carboxylic acid synthase) gene.

Using quantitative real-time PCR (q PCR), we previously confirmed that the ACS gene was upregulated in poplar (Populus trichocarpa) plants when exposed to environmental pollutants including ozone and methyl vinyl ketone.

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The recombinant plasmid containing the ACS gene was electroporated into the GV101 strain of Agrobacterium tumefaciens.

Arabidopsis plants were genetically engineered with ACS gene by dipping the T0 (transgenic zero or wild type) flowering plants into GV101 strain of Agrobacterium containing the recombinant binary vector.

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